Leica Biosystems CM1950 - Manual (Page 34)

34 Instructions for Use V 1.5 RevF - 08/2014
• The Peltier element provides additional cooling for the freezing stations.
After the button is pressed, the display changes from "PE" to "10", indi-
cating an additional cooling period of 10 minutes. The countdown of the
remaining cooling time is permanently displayed. Once only 4 minutes
are remaining, a dot will appear after the "4". As of this time, the Peltier
element may be switched off early by pressing the button again.
• Press the "VAC" button to enable the vacuum extractor. The LED in the "VAC"
button is lit while the extractor is on. Press the button again to disable it.
• Use the knob to adjust the intensity of the vacuum.
A Optimal area for trimming and sectioning
B Optimal area for extraction from the chamber
• To clean the chamber, turn the knob to the red range.
7. Instrument Controls
Position
12 o'clock
6 o'clock
A
B
Fig. 35
Fig. 36
• Trimming: Handwheel position12 – 6 o'clock, valve open
Handwheel position 6 – 12 o'clock, valve closed
• Sectioning: Handwheel position12 – 3 o'clock, valve open all the way
Handwheel position 3 – 6 o'clock, valve half open
Handwheel position 6 – 12 o'clock, valve closed
The strength of the required extraction force depends on the following:
• Size of the specimen
• Sectioning speed
• Section thickness used
Caution:
The specimen head and Peltier do not switch on until the chamber
temperature reaches -5 °C, in order to prevent icing.
If the condenser (resting phase) is off and the Peltier cooling is acti-
vated, the number 10 flashes; until the condenser switches on again
to prevent the Peltier from being destroyed when the condenser is
not running. When the condenser starts up, the flashing starts and
the 10 minutes are counted down.
(For exact instructions for using the chamber, specimen head and
real time display fields, refer to the chapter on "Working with the
Instrument" .)