Leica Biosystems CM1950 - Manual (Page 38)

38 Instructions for Use V 1.5 RevF - 08/2014
7. Instrument Controls
Disinfection
Duration - 30 min
Duration – 180 min
UVC key - to activate / deac-
tivate the disinfection cycle
and/or to acknowledge in-
terruption of a disinfection
cycle.
To start disinfection, the sliding window must be
completely closed.
• Press UVC key once briefly to start the 30 min. mode
• UVC button – press 1x for a longer time (approx.
4 sec), 180 min mode
Fig. 39
For current information about certificates and
recommendations, visit:
http://www.leicabiosystems.com/specimen-
preparation/sectioning/cryosectioning/details/
product/leica-cm1950/downloads/
* Leica Cryofect is not available in all countries.
UVC disinfection is effective when disinfecting surfaces and air within the irradiated working area
of the Leica CM1850 UV, CM1900 UV and CM1950 cryostats at -20 °C (Table 1, see Certificate I. Maier ).
For powerful disinfection, we recommend irradiation for three hours (CM1850 UV/CM1950) and four
hours (CM1900 UV). Vegetative bacteria including Mycobacterium tuberculosis, bacterial endospores
(Bacillus sp.) and fungi are killed during this time. Viruses, including such resistant species as, for
example, hepatitis viruses, are also deactivated to at least 4 log10 units (99.99 %).
Medium disinfection can be attained through short irradiation for 30 minutes (CM1850 UV/CM1950)
and 40 minutes (CM1900 UV). This reduces vegetative bacteria including Mycobacterium tuberculosis
and sensitive viruses such as the influenza A virus (also including the highly pathogenic avian influ-
enza virus H5N1) and the polio virus by at least 5 log10 units (99.999 %).
UVC irradiation within the working area of the cryostats can provide reliable and efficient disinfec-
tion of surfaces and the air and significantly reduces the risk of infection.
We recommend wiping off visible contamination in the cryostat with an alcohol-based disinfectant
prior to using the UV lamps. The germicidal effect of the irradiation is restricted to the directly irradi-
ated areas, which is why UVC irradiation cannot be a replacement for regular chemical disinfection
of the cryostat chamber.
Specimens and section waste must be thoroughly removed from the cryochamber first (e. g. using the
vacuum extractor (optional), or a paper towel soaked with Cryofect* or alcoholic disinfectant). Be-
fore UV disinfection, move the anti-roll guide to the side to allow complete disinfection.
Opening the sliding window cancels the disinfection cycle. Press the UVC key to acknowledge this.
When the keypad lock is activated (via the key button) the UV lamp can be shut off only by opening
the glass, as the UV keys are locked.
The cancellation can be acknowledged only if the keypad lock is disabled. Only then can the UV
lamp be switched back on.