Leica Biosystems CM1950 - Manual (Page 43)

43 Leica CM1950 – Cryostat
8. Working with the Instrument
Manual defrosting of the specimen cooling
• Push the manual defrost button (with the melting snowflake) on the left
over the key button to activate the defrost cycle of the specimen head.
• Activation is confirmed by an audible signal.
• Then, push the "+" or "-" button on the panel for the specimen tempera-
ture.
• There is a flashing indication of the specimen temperature during the
defrost cycle.
• For 15 minutes, the specimen head is adjusted to a temperature of 45 °C.
• Subsequently, the instrument adjusts to the specimen temperature which
has been programmed prior to the manual defrost cycle.
• If necessary, push the manual defrost button again to deactivate the
manual defrost cycle.
Fig. 47
Fig. 48
Press the key button, then the "+" button = specimen head on
Press the key button, then the "-" button = specimen head off
Before starting the defrost cycle, remove all specimens from the
cryochamber!
Manual defrosting of the cryochamber
• Push the manual defrost button (with the melting snowflake) on the left
over the key button to activate the defrost cycle of the cryochamber on
demand.
• Activation is confirmed by an audible signal.
• Then, push the "+" or "-" button on the panel for the cryochamber tem-
perature.
• The manual defrost cycle (12 min.) is activated.
• There is a flashing indication of the temperature of the cryochamber
during the whole defrost cycle.
• If necessary, push the manual defrost button again to deactivate the
manual defrost cycle.
• When the manual defrost begins, the specimen head adjusts to a tem-
perature between -10 °C and -5 °C (reduction of ice formation). The
specimen head cooling turns off. This is confirmed by the flashing of the
decimal points on the panel for the specimen cooling.
• Ten seconds after the manual defrost cycle has been completed, the
specimen cooling turns back on.
Fig. 49