Leica Biosystems CM1950 - Manual (Page 48)

48 Instructions for Use V 1.5 RevF - 08/2014
E
8. Working with the Instrument
• Check that the suction nozzle is seated optimally (by gently turning the
handwheel)
• Activate trimming mode.
• Select the trimming thickness.
• Switch on the VAC and select a low extraction level (between 1 and 2).
• Start trimming by manually moving the handwheel or press the
RUN/STOP and ENABLE buttons simultaneously to start motorized sec-
tioning.
• Use a brush to apply the section to a precooled specimen slide, then
warm it from below using your finger.
• Move the knife guard forward after removing the section.
• Remove the knife or disposable blade (using the blade ejector!).
• Insert the knife into the knife case.
The tension acting on the hose can be minimized by turning the red ring
( Fig. 28 ) clockwise so that the suction nozzle presses against the pres-
sure plate.
Fig. 59
Trimming with extraction – 2. Brush technique, finger rest installed
• Remove the silicone stopper (E) from the filter cover (and keep it in a
safe place).
• Insert the extraction hose with the black adapter.
• Fasten the suction nozzle to the pressure plate (using 4 magnets on the
rear side of the nozzle) as far as possible towards the blade.
If the handwheel is not moved for approx. 5 sec, the valves close and the fan remains on.
If the handwheel is moved for approx. 1 min, the valves close, the fan shuts off (the LED
in the VAC switch goes off to prevent icing).
To continue working, you now have to reactivate the VAC button.
Ensure that the hose with the nozzle is not installed against its "natural" curvature on the
pressure plate of the blade holder.
Fig. 60