Leica Biosystems CM1950 - Manual (Page 46)

46 Instructions for Use V 1.5 RevF - 08/2014
8. Working with the Instrument
8.4.2 Trimming with extraction – 1. Anti-roll guide installed
• Remove the silicone stopper from the filter cover (and keep it in a safe
place).
• Insert the extraction hose with the black adapter.
• Fold the anti-roll guide to the side and fasten the extraction nozzle to
the pressure plate (using 4 magnets on the rear side of the nozzle) – see
mark ( Fig. 56a ) – (using 4 magnets on the rear side of the nozzle).
• Fold the anti-roll guide back into position.
E
Fig. 56a Fig. 56b Fig. 56c
• Approach the specimen with the knife or blade holder:
• To do so, open the clamping lever of the base, approach the specimen
and close the lever again.
• Open the lever of the orientation. Orient the specimen (move it into a
favorable position relative to the knife/blade) and close the lever again.
• Approach the knife or blade holder using the coarse feed buttons and
gentle movements of the handwheel.
Check the stability of the specimen head each time it clicks back into place. This can
prevent it from becoming difficult to set the zero position.
Before using them for the first time, degrease new knives using
acetone or alcohol.
If the sections are cracked, the specimen head temperature is too
cold. Set a warmer temperature.
If the sections smear, the specimen head temperature is too warm.
Set a colder temperature.
Fig. 55
Fig. 56