Leica Biosystems CM1950 - Manual (Page 53)

Leica CM1950 – Cryostat 53
9. Troubleshooting
Problem Cause Remedy
Blunt knife
Inappropriate section thickness.
Incorrect clearance angle.
Microtome not dried thoroughly
enough.
Dried specimen.
Use different part of the knife.
Select correct section thickness.
Set correct angle.
Dry microtome.
Prepare new specimen.
Tissue sticks to the anti-roll
plate
Anti-roll plate is too warm or
incorrectly positioned.
Fat on the corner or edge of anti-roll
plate.
Anti-roll plate not correctly fixed.
Rust on the knife.
Cool down anti-roll plate, or
reposition correctly.
Remove fat from anti-roll plate.
Fix correctly.
Remove rust.
Flattened sections curl
up when anti-roll plate is
folded up
Anti-roll plate too warm. Cool down anti-roll plate.
Sections tear or separate Temperature too low for the tissue
cut.
Blunt part, dirt, dust, frost or rust on
the knife.
Top edge of the anti-roll plate
damaged.
Hard particles in the tissue.
Dirt on knife back.
Set alternative temperature and
wait.
Remove cause.
Replace anti-roll plate.
- - -
Clean.
Cryostat non-operational Power plug not properly connected.
Defective fuses, or circuit breaker has
triggered.
Check if properly connected.
Replace fuses, or switch circuit
breaker back on. If not possible, call
technical service.
Specimen disc cannot be
removed
Moisture on the underside causes
the specimen to freeze to the freezing
shelf or specimen head.
Apply concentrated alcohol to the
contact point.