Leica Biosystems CM1950 - Manual (Page 50)

50 Instructions for Use V 1.5 RevF - 08/2014
8. Working with the Instrument
After trimming or sectioning:
Specimen:
• Unclamp and thaw.
• Immerse in fixative for further processing.
Cleaning
• Use the brush to sweep up the section waste (section waste tray) and
remove it from the cryostat (follow applicable laboratory regulations for
disposal).
or
• Clean the cryostat chamber using the chamber suction nozzle:
• To do so, turn the (flat) suction nozzle of the extraction hose by
holding the hose on the white adapter and removing it with a
quick twist. "Park" the flat suction nozzle in a designated place in
the chamber – e.g. on the right inside wall of the cryochamber.
• Remove the chamber suction nozzle from the plastic clip and
attach it firmly to the white adapter.
• Check the remaining capacity of the filter (in
the interior of the chamber) and change the
filter if necessary ( see Chap. 6.5.8 ).
• Check the bacteria filter (in the front of the
unit, ( see Chap. 6.5.7 ), change it at least every
3 months.
• Move the anti-roll guide to the side ( see
Fig. 61 ).
• Spray the cryostat chamber using Cryofect.
• Activate the UVC disinfection.
The use of flammable sprays within the
cryostat chamber
is permitted only with the Leica Cryofect
tested by us.
Fig. 61