Leica Biosystems CM1950 - Manual (Page 49)

49 Leica CM1950 – Cryostat
8.4.3 Cutting with extraction – Anti-roll guide installed
• Shut off VAC (LED in VAC button goes out).
• Switch from trimming to sectioning mode (important for section stretch-
ing, as the valves work differently than in trimming mode).
• Set the desired section thickness.
• Switch on the VAC and begin at level 1. If the section does not stretch
correctly, turn the VAC knob higher in small increments.
• Once the desired section is on the pressure plate, switch off the VAC!
Carefully fold the anti-roll guide to the side and remove the section from
the side.
A few rules:
• Always begin at a low extraction level, then slowly increase it.
• Do not use high extraction levels unless absolutely necessary.
• Different specimen sizes require different extraction levels.
• The faster the trimming or sectioning speed, the lower the extraction
level should be.
• The larger and/or thicker the specimen to be trimmed, the lower the
extraction value.
• For section specimens with a diameter of 0.5 cm, the anti-roll guide
stretches the section adequately. For larger specimens, we recommend
using the vacuum function.
8. Working with the Instrument
Sectioning with extraction without anti-roll guide (brush tech-
nique) is not possible, as the position of the pressure plate means
that no suitable air flow is obtained.
After removing the section, wipe off moisture/condensate from the
pressure plate – otherwise, the next sections will become jammed.