Leica Biosystems CM1950 - Manual (Page 47)

47 Leica CM1950 – Cryostat
8. Working with the Instrument
• Optimize the vacuum settings if necessary.
• Press the "VAC" button to enable the vacuum extractor. The LED in the
"VAC" button is lit while the extractor is on. Press the button again to
disable it.
• Use the knob to adjust the intensity of the vacuum.
A Optimal area for trimming and sectioning
B Optimal area for extraction from the chamber
• To clean the chamber, turn the knob to the red range.
A
B
The tension acting on the hose can be minimized
by turning the red ring ( Fig. 57 , top right) clock-
wise so that the suction nozzle presses against
the pressure plate (I, Fig. 57 ).
A
D
I
B
C
Fig. 57
K
• Check that the anti-roll guide is parallel and correctly adjusted. Readjust
if necessary ( see page 62 ).
• Activate trimming mode.
• Select the trimming thickness.
• Switch on the VAC and select a low extraction level (between 1 and 2).
• Start trimming by manually moving the handwheel or press the
RUN/STOP and ENABLE buttons simultaneously to start motorized
sectioning.
Fig. 58
• Trimming: Handwheel position 12 – 6 o'clock, valve open
Handwheel position 6 – 12 o'clock, valve closed
• Sectioning: Handwheel position 12 – 3 o'clock, valve open all the way
Handwheel position 3 – 6 o'clock, valve half open
Handwheel position 6 – 12 o'clock, valve closed
Ensure that the hose with the nozzle is
not installed against its "natural" curva-
ture on the pressure plate of the knife
holder.
For motorized sectioning, always begin at low speed for safety
reasons.