Metrohm 820 IC Separation Center - Manual (Page 48)

2 Installation
819 IC Detector / 820 IC Separation Center
40
53 Separating column
56 Detector block
57 Inlet capillary for detector block
fixed mounting
58 Mounting rail
59 Column holder (6.2027.0X0)
60 Capillary for syringe 13
PEEK capillary, fixed mounting
61 Sample loop (6.1825.210)
20 µ L, PEEK, fixed mounting
62 Inlet capillary for injector
PEEK-Capillary 6.1831.010
63 PTFE aspirating tube
fixed mounting
With the one-channel system without suppressor module, the IC sepa-
rating column is installed in the 2.820.0X10 IC Separation Center as fol-
lows (see Fig. 21):
1 Connect column to injector
• Remove end caps from column 53.
• without column heating:
Screw inlet end of separating column 53 (note flow direction)
to column connection capillary 50 mounted on the injector.
• with column heating:
Prepare column heating according to Section 2.9.2 and
screw column connection capillary 50 (see Fig. 20) with a
compression fitting to injection valve 51.
• With precolumn:
Install precolumn according to the supplied leaflet between
inlet of the separating column and the injection valve.
2 Rinse column
• Place a beaker beneath the column outlet.
• Start 818 IC in «IC Net» with suitable flow (see leaflet of the
column) and rinse column for ca. 10 min with eluent.
• Stop 818 IC Pump.
3 Connect column to detector block
• without column heating
Screw outlet end of separating column 53 to the inlet capillary
57 permanently mounted on the detector block 56.
• with column heating
Connect outlet end of separating column 52 (see Fig. 20) with
coupling (6.2744.040) to the inlet capillary 57 permanently
mounted on the detector block 56.
4 Fix column
• without column heating
Insert one or two column holders 59 (6.2027.030, 6.2027.040
or 6.2027.050) in the mounting rails 58 and fasten separating
column in the column holder.
• with column heating
Insert column heating according to Fig. 10 into the Separation
Center.