Metrohm 820 IC Separation Center - Manual (Page 80)

4 Notes - Maintenance - Faults
819 IC Detector / 820 IC Separation Center
72
5 Remove coupling
• Remove 6.2744.040 Coupling 71 between column connec-
tion capillary 50 and inlet capillary 57 (see Fig. 21).
6 Connect new column to injector
• Remove end caps from column 53.
• Screw inlet end of separating column 53 (note flow direction)
to column connection capillary 50 or to the precolumn (see
Section 2.9.5/Section 2.9.7).
7 Rinse column
• Place beaker beneath the column outlet.
• Switch on 818 IC Pump and rinse column with eluent for ca.
10 min, then switch off pump.
8 Connect column to detector block
• Screw outlet end of separating column 53 to inlet capillary 57
or suppressor inlet capillary 64.
4.2.6 Regeneration of suppressor
Regenerating a suppressor with reduced capacity
If the suppressor units are exposed to certain heavy metals (e.g. iron)
or organic contaminants for long periods of time, these can no longer
be completely removed by the regeneration solution normally used (20
mmol/L H
2
SO
4
). This diminishes the capacity of the suppressor units,
which, in milder cases, results in a reduced sensitivity to phosphates
and, in severe cases, in a strong increase in the baseline. If such ca-
pacity problems occur at one or several positions, the suppressor units
must be treated as follows:
1 Disconnect suppressor from IC system
• Disconnect suppressor from separating column and detector.
2 Regenerate suppressor
• Rinse each suppressor unit for about 10 min with one of the
following solutions:
Contamination with heavy metals
0.2 mol/L H
2
SO
4
+ 0.1 mol/L oxalic acid (with Fe 2+ /Fe +3 )
Contamination with organic substances
0.2 mol/L H
2
SO
4
/ acetone ≥ 20%
The 6.1826.050 pump tubing is made of PVC and must
not be used for rinsing with solutions which contain ace-
tone. In such cases, rinse with different pump tubing or a
different pump.