Oritron Dionex Ion Chromatography System ICS-2100 User Manual

Page 274

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ICS-2100 Ion Chromatography System

262

Doc. 065291-01 3/09

Packing
The material that fills a chromatographic column; usually a resin or silica-based
material.

Pellicular Resin
A resin with a solid, nonporous core coated with a thin layer of more porous
material. The exchange sites of pellicular ion exchange resins are located only on
the surface layer of the bead. These resins have a low ion-exchange capacity.

Pre-Column
Synonymous with Guard Column.

Regenerant
A dilute acid or base that converts ion exchange sites in a MicroMembrane
Suppressor back to the form that suppresses the eluent conductivity.

Resin
See Ion-Exchange Resin.

Resolution (R)
A measure of the separation between two sample components. This is expressed
as the ratio of the distance between the two peak maxima to the mean value of the
peak width at the baseline.

R = 2(t

2

- t

1

)/(W

2

+ W

1

)

Where: t

1

and t

2

=the retention times of components 1 and 2,

respectively
W

1

and W

1

= the baseline width of peaks 1 and 2,

respectively (measured in the same units as the retention
time)

R is proportional to the square root of efficiency (N). A value of R = 1.5
represents “baseline separation” of the two peaks.

Retention Time
The time from injection to peak maximum; the basis for identification of a species
in chromatographic analysis.

Separator Column
The column used to perform a chromatographic separation; also called an
analytical column.

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