Operation – Leica Biosystems CV5030 User Manual

Page 68

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Instructions for Use V 3.0 RevE – 04/2013

5. Operation

5.13.1 Procedure (continued):

If artifacts become visible within the monitoring

process (streaks, cloudiness, discoloration), the

involved reagents must be inspected for compat-

ibility and storage life.

The staining protocol should be adapted as

needed.

7. Inputting the first test parameters:

7 a) Effect of parameters on the application

quantity

• STK - Stroke length of the coverslip mountant

corresponding to the coverslip length used.

• VOL - Quantity of the coverslip mountant:
1 (min.) -> 9 (max.)
• TYP - Pressure in the mountant bottle:
1 (min.) -> 10 (max.)

7 b) Guideline for low-viscosity coverslip moun-

tants:

Dispenser needle: 20 or 21 (smallest diameter)

Menu A:
• STK: Adjust stroke length to the cover slip

length used

(e.g.

STK = 50 for coverslip size 22 x 50 mm and

24 x 50 mm).

• VOL: Start the first coverslip trials with VOL set

low (approx. 2-4)

• TYP: Start the first coverslip trials with TYP set

low (approx. 2-4)

The parameters VOL and TYP are incrementally

adjusted (increase or reduction) until a suitable

application quantity on the specimen slide is at-

tained. Further procedures are described in the

following

Point 8, "Fine-tuning"

7 c) Guideline for high-viscosity coverslip moun-

tants

Dispenser needle: 18 or 16 (largest diameter)

Menu A:
• STK: Adjust stroke length to the cover slip

length used

(e.g.

STK = 50 for coverslip size 22 x 50 mm and

24 x 50 mm).

• VOL: Start the first coverslip trials with VOL

(approx. 5-7)

• TYP: Start the first coverslip trials with the

setting TYP (approx. 5-7)