Labconco Type C1 Biosafety Cabinet Models 30468 - Manual (Page 80)

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Exhaust HEPA Filter Leak Test
All Axiom models were tested to the HEPA Filter Leak Test as described in ANSI/NSF
Standard 49:2012.
Note: ensure all internal surfaces of the Axiom have been appropriately
decontaminated before proceeding.
Note: For all Axiom models, the work surface may be removed if desired, and
the aerosol generator discharge should be placed in the center exhaust duct
to inject the aerosol as shown in Figure 5-7.
Figure 5-7
Testing at Labconco has shown the actual concentration varies from the calculated
value. Table 5-2 shows the actual vs. calculated concentrations for the models.
Table 5-2
Exhaust HEPA Filter Leak Test Specifications
Model 3044- 3046-
Air Displacement (CFM) 723 1083
Laskin Nozzles needed 2 2
Theoretical aerosol conc.
(ug/l)
1
38 25
Actual aerosol conc. (ug/l)
1
21 17
3. The calculated concentration was established by the formula:
Concentration (ug/l) = (# Laskin nozzles @ 20 PSIG x 13,500)/Volume of air displaced
4. Based on Mineral oil
1. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the “CLEAR” setting.
2. Check the oil level of the generator – it must be within 1/8” of the level line
when the generator is sitting level.
3. Establish the 100% and 0% concentration levels for the photometer.