Metrohm 788 IC Filtration Sample Processor - Manual (Page 39)

2.4 Installation for full control with «IC Net»
788 IC Filtration Sample Processor
31
Settings in the «IC Net 2.1» program
There are two ways to operate an automated Metrohm IC system with
dialysis:
A) For each sample, every analysis procedure contains first a dialysis
step followed by the chromatographic separation.
Ÿ The total analysis time per sample results from summation of
the single times of dialysis and separation.
B) Chromatographic separation of one sample and dialysis of the next
sample is done simultaneously.
Ÿ The total analysis time per sample is reduced to the time of
chromatographic separation.
Both techniques are described on the following pages.
A) Dialysis and separation of one sample in a single procedure
1. Definition of new program parameters for the 788 IC Filtration
Sample Processor under Configuration/Control:
PUMP 754 on = ---****1******
PUMP 754 off = ---****0******
2. Program for the 788 IC Filtration Sample Processor:
001 Move sample
002 Lift work
003
Ctrl PUMP 754 on
004 Wait 120 s
005 Ctrl FILL B 1 / STEP
006 Wait 480 s
007 Ctrl ZERO 1
008 Ctrl STEP MSM 753
009 Wait 120 s
010 Ctrl FILL A 1
011 Ctrl INJECT B 1
012 Wait 30 s
013 Ctrl INJECT A 1
014 Ctrl PUMP 754 off
 Move needle to sample position
 Place lift with needle to working position
 Start 754 Pump Unit
 Rinse dialysis cell with sample solution
 Stop of acceptor solution
 Dialysis time
 Trigger autozero at 732 IC Detector
 Switch 753 suppressor module to next position
 Additional dialysis time (total 600 s)
 Switch injection valve A at 733 to "Fill"
 Transfer acceptor solution to sample loop
 Fill sample loop with acceptor solution for 30 s
 Switch injection valve A at 733 to "Inject"
 Stop 754 Pump Unit