Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual

Page 103

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Protocol Window

91

E

NTERING

C

ONCENTRATIONS

(

FOR

C

URRENT

S

TANDARDS

O

NLY

)

To calculate them automatically from a dilution series, select Enter
Automatically.

Enter the high standard concentration (S1) into the first row of the table, for
each analyte. Then select the Dilution Factor, check the Apply dilution to all
analytes box, and click Calculate. The table fills with the standard
concentrations.

If you are using external standards, the concentrations are automatically
entered, and you can skip to the next section on selecting concentration units
(page 93). To enter concentrations, make sure the Standards Info tab is
selected.

E

NTERING

C

ONCENTRATIONS

M

ANUALLY

You can enter your concentrations manually. The table lists your standard
wells (S1, S2, S3, etc.) These are the wells that you defined in the Format
Plate window (page 68). For the selected analyte, enter the concentration for
each standard in the concentration column.

Figure 82. Entering standard concentrations manually

If the Apply same concentrations to all analytes checkbox is selected, the
concentration values you enter are applied to all the analyte standards.
Deselect the checkbox if you want to enter different concentrations for each
analyte standard.

You can also enter additional information about your standards in the
description column for each well.

NOTE: You can use the Cut, Copy, and Paste commands on the Edit menu to
copy your descriptions and concentrations among analytes. You can also
copy and paste from and to other applications, such as Microsoft Word or
Excel. Click a cell, row, or column in the table to copy and paste.

If you are entering different concentrations for each analyte standard, repeat
the procedure by selecting each analyte from the pulldown menu as described
above.

BioPlex_6.book Page 91 Friday, September 23, 2011 11:55 AM

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