Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 120)

Bio-Plex Manager Software 6.1 User Guide | Running Protocols
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WARNINGS:
• Protect your assay microspheres from light. Once photobleached,
the beads are no longer usable. Proper care of microspheres must
be observed to maintain the benefits of your product warranty
• When using a filter plate, avoid touching the bottom of the wells
when wrapping the plate. Sample may wick or leak from the
bottom of the well, causing problems when reading the plate
• Make sure that you have added at least 125
l of sample to all the
wells specified in your plate template before starting a reading. If
the array reader attempts to draw sample from an empty well, air is
sucked into the sample loop and injected into the flow chamber. As
a result, bubbles form in the cuvette and interfere with the analysis.
If this happens, perform a Remove Air Bubbles procedure
(page 39) and rerun the Protocol
• Shake the microplate on a plate shaker for 30 seconds prior to
performing a reading
Running the Protocol
1. When you have selected all of your Protocol options and prepared your
microplate, click Start.
The microplate platform automatically ejects.
NOTE: If you are running the Security Edition, you are prompted
to enter your Electronic Signature after you click Start.
Figure 97. Run Protocol dialog
2. Place the microplate on the microplate platform.
BioPlex_6.book Page 108 Friday, September 23, 2011 11:55 AM