Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 116)

Bio-Plex Manager Software 6.1 User Guide | Running Protocols
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Standard Curve Optimization
When Protocols are run, by default, the software will optimize the fit of the
standard curves. This can be turned off from the Run Protocol window, if
optimizing is not desired. In Security mode, only users with permissions are
allowed to change this setting. For more information on standard curve
optimization see Standard Curve Optimizer on page 199.
Advanced Run Settings
Click the Advanced Settings button to access additional reader settings and
controls.
Figure 95. Advanced Run Settings dialog
Bead Map Selection
Bio-Plex Manager software identifies each bead in an assay by its
classification region—that is, the region in the bead map where the bead falls,
based on its fluorescence as measured by the classification channels in the
array reader (see page 115). The default bead map is the 100-region map,
which is appropriate for assays that use xMAP microspheres (such as
Bio-Plex assays, most Bio-Plex Pro assays, and Bio-Plex COOH beads). A
25-region map is also available.
The 25-region map includes select regions from the default 100-region map.
The result is that the regions are larger and more spread out. The 25-region
map is not available when creating a new panel, as it applies only to a specific
subset of pre-installed Bio-Rad assays. It will be applied correctly to these
preinstalled assays.
BioPlex_6.book Page 104 Friday, September 23, 2011 11:55 AM