Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 105)

Protocol Window
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C ONCENTRATION U NITS
You can enter the units of concentration in the Concentration Units field (units
are not required and do not affect calculations). These units appear in your
reports. If all your analytes have the same concentration units, select the
Same Units for All Analytes checkbox.
R EGRESSION M ETHODS
Using the Std Regression Curve pulldown list, choose from among seven
different regression methods to generate the standard curve (page 168):
• Logistic-5 PL
• Logistic-4 PL
• Linear
• Cubic spline
• Point to point
• Linear (semi-log)
• Point to point (semi-log)
Figure 84. Selecting a regression method
You can change your selection later in the Standard Curve window of the
Results file. See page 170 for a more detailed discussion of regression
methods.
You can also select the regression-curve axis scale from four different
combinations of linear and logarithmic scales. Select the axis scale from the
Axis Transformation pulldown list.
Figure 85. Selecting a regression-curve axis transformation
Note that the axis transformation only changes how the curve is displayed,
not the calculated values.
NOTE: The regression method and axis transformation you select in the
Protocol window is used to calculate your initial results. However, these
settings do not affect the reading, and you can change these settings and
recalculate your results after a reading is complete.
Finally, if you want to use different regression methods for different analytes,
deselect the Same Regression Type for All Analytes checkbox. Then, when
you select a different analyte from the pulldown list, you can select a new
regression method as well.
BioPlex_6.book Page 93 Friday, September 23, 2011 11:55 AM