Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 182)

Bio-Plex Manager Software 6.1 User Guide | Analyzing the Results
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Regression Methods
There are seven regression types for calculating the standard curve:
• Logistic-5PL
• Logistic-4PL
• Linear
• Cubic spline
• Point-to-point
• Linear (semi-log)
• Point-to-point (semi-log)
The minimum number of nonzero standards required for each regression
method type is shown in the following table.
Figure 155. Example of two Logistic-5PL curves, for standards on the current
plate, and external standards
If you do not have the minimum number of standards for the selected method,
an error message will be displayed in red below the standard curve.
Method Minimum number of standards
Logistic-5PL 6
Logistic-4PL 5
Cubic spline 4
Linear (linear and semi-log) 2
Point to point (linear and semi-log) 2
BioPlex_6.book Page 170 Friday, September 23, 2011 11:55 AM