Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 228)

Bio-Plex Manager Software 6.1 User Guide | Troubleshooting
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“Bio-Plex Manager has
detected a possible
problem due to
microbubbles in the
cuvette or
photobleached beads” More likely:
Calibration was performed
before the array reader was
warmed up. Perform 30-minute warm up and
recalibrate.
Calibrated with incorrect CL1 or
CL2 target values. Check that the target values of the CAL
beads match values entered in the
software. If different, recalibrate.
Array reader was calibrated with
a dirty MCV Plate IV. Clean MCV Plate IV and recalibrate.
Misalignment of optics. Perform Optics Validation. Contact
Technical Support if values are not
within range.
Less likely:
Microbubbles present in cuvette. Perform Remove Bubbles.
Calibration beads are
photobleached (do not expose to
light for more than 1 hour). Recalibrate with new CAL1 beads.
Needle stuck in down
position Protective assay plate covering
was not removed. See hardware manual for procedure for
raising needle stuck in down position.
Then remove cover from assay plate.
Needle guide is not screwed all
the way in. Tighten needle guide by turning tube
clockwise until tight.
Sample needle is bent. Replace bent needle with a new needle
(see hardware manual for procedure).
Optics, Fluidics,
Reporter, or Classify
Validation Procedure
fails due to low bead
number Beads not resuspended. Resuspend beads and repeat validation
procedure. If procedure still fails,
contact Technical Support.
Clog in instrument. Perform Unclog operation and repeat
validation procedure. If procedure still
fails, contact Technical Support.
“Bio-Plex Manager has
detected a problem
with bead
aggregation” Incorrect DD gate values used. View histogram in Run Protocol window
and make sure DD peak falls within the
gate range. If necessary, adjust gates.
Clumped beads present. Vortex plate at 900 rpm for 11 minutes.
Sheath reservoir is empty. Refill sheath reservoir, then perform
Start Up.
Waste reservoir is overfilled. Empty waste and reconnect.
Incompatible suspension buffer
used. Check hardware manual for buffer
compatibility.
Message/Problem Cause Solution
BioPlex_6.book Page 216 Friday, September 23, 2011 11:55 AM