Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 45)

Calibration
33
Setting Up the Calibration
When you have specified the control numbers of your calibration
microspheres and specified the type of calibration you want to perform (CAL1
& CAL2, CAL1 Only, or CAL2 Only), click OK in the Calibrate dialog.
Another dialog box lists step-by-step instructions for preparing the Bio-Plex
MCV Plate IV.
Figure 19. Preparing the Bio-Plex MCV Plate IV for CAL1 & CAL2 calibration
NOTES: When preparing the MCV Plate IV, do the following:
• Important: Before vortexing, remove the calibration beads from 2 to
8°C (36 to 46°F) storage, and allow them to warm to room
temperature. Vortex each bottle for 30 seconds. Proper suspension
of the microspheres is essential for efficient calibration
• Never dilute the calibration beads, and be careful to limit their
exposure to light. Store at 2 to 8°C immediately following
calibration
• Load 6 drops of beads (approximately 200 μl) per reservoir (CAL1
or CAL2)
• The DI H
2
O well of the MCV Plate IV holds about 3 ml of distilled or
deionized water
BioPlex_6.book Page 33 Friday, September 23, 2011 11:55 AM