Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 122)

Bio-Plex Manager Software 6.1 User Guide | Running Protocols
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Status Bar
As the sample is analyzed, the status of the reading displays in the status bar
at the bottom of the Bio-Plex Manager window.
Figure 98. Status bar during a reading
The status bar provides a running count of both the Number of beads and the
Beads per second. Within these categories, the array reader keeps track of
the following information:
• The Region fields monitor only those beads that fall within one of
the regions you selected in the Protocol (page 60)
• The Gate fields monitor all beads that pass an internal
discriminator gate (DD gate range) designed to eliminate particles
smaller or larger than a microsphere (including microspheres that
are clumped or aggregated)
• The Total fields report all particles measured by the array reader,
including those that do not pass the internal discriminator gate
Manually Stopping a Reading
While the sample is being read, the Start button changes to a Stop button in
the Protocol window. You can click Stop to cancel the reading. An alert box
notifies you that you can generate a report with the incomplete results, or
rerun the Protocol as described on page 123.
Generating Results from a Protocol
The reading stops when you click Stop, when an error stops the reading, or
when all samples have been analyzed. At the end of the reading, the sample
needle lowers and the syringe pump purges the sample loop and needle using
sheath fluid. Then it sends a fraction of sheath fluid (approximately 160
l)
back into each well.
At the end of a complete reading, the Results are automatically calculated and
displayed.
Note that the Results file automatically is saved if you selected Auto Save
After Run (page 105) in the Protocol. Otherwise, you must save your Results
as a separate step (see Saving a Results File on page 144).
BioPlex_6.book Page 110 Friday, September 23, 2011 11:55 AM