Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 250)

Bio-Plex Manager Software 6.1 User Guide | Glossary
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PE See phycoerythrin.
Phosphoprotein An enzyme that functions to phosphorylate specific proteins.
Phosphoproteins are responsible for the activation of a variety of
proteins through the process of phosphorylation.
Photobleaching A chemical reaction caused by exposure to light, in which a
fluorochrome is converted into a differently fluorescent or non-
fluorescent compound.
Photomultiplier tube A light detector typically used in fluorescence detection systems,
designed to convert a fluorescent signal into an electronic signal that can
be quantitated. PMT settings display as an RP1 number during
calibration, when using Bio-Plex Manager.
Phycoerythrin (PE) The fluorochrome used as the reporter molecule in Bio-Plex assays.
Excitation = 546 nm, emission = 575 nm.
PMT See photomultiplier tube
Precision A measure of the reproducibility of replicate readings, usually
represented by the coefficient of variation (%CV).
Protocol file (*.pbx or
*.spbx) A file containing the settings of a reading, including the microplate wells
to read, the analytes to detect, sample size, etc. To perform a reading,
you open a Protocol file, select the settings, and then run the Protocol.
You can then save the Protocol settings and reuse or modify them. After
a reading, a Results file is created containing the settings from the
Protocol as well as the data results. The raw data from the most recent
reading are also stored in the Protocol file. In Bio-Plex Manager Security
Edition, Protocol files may be Secure files, in which case they have the
file extension *.spbx.
Quality control Procedures and guidelines that determine conformity to requirements.
Recovery percentage A mechanism for assessing the fit of the standard curve to the actual
standards. For each analyte standard, an observed concentration is
back-calculated from the standard curve and the fluorescent intensity.
This is divided by the expected concentration and multiplied by 100 to
give the recovery percentage.
Recovery range The range of acceptable recovery percentages (see above). For
example, a recovery range of 80–120% means that the observed
concentration of a standard should be within 80–120% of the expected
concentration. Concentrations of standards and unknowns that fall
outside this range are flagged as unreliable.
Reference A well within a group of wells defined on a plate that is used as a
reference. You can calculate the ratio of the fluorescent intensity of the
Reference well to the fluorescent intensities of the other wells in the
group.
Region The region of a fluorescent color map used to identify a particular bead
set. Each bead set is embedded with specific quantities of two
fluorescent dyes; the combination of these fluorochromes, as detected
by the array reader, places the bead set within a unique region on the
color map, thereby identifying the set and its associated analyte.
Term Definition
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