Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 213)

Determining Outliers
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Concentration in Range
Concentration in range (Conc in Range) reports which standards, controls,
and unknown concentrations are reliable, based on the recovery range of the
standards. The Conc in Range column shows only observed concentration
values that fall within the range of valid standards, as defined by acceptable
recovery ranges. Values that do not fall in the range are displayed as < OOR or
OOR >.
Figure 186. Concentration in Range and Observed Concentration columns
Determining Outliers
The Standard Curve Optimizer maximizes the usable range of an analyte's
standard curve, based on the percent recovery range selected (the
recommended recovery range is 70-130%). The optimization program
evaluates several parameters, including tests for bead count, CVs of
replicates
hook effect, recovery value and points in the noise region of the
curve (flatness at the low end).
Additionally, the optimization program will favor the fit at the low or high end of
the curve, depending upon the number of unknowns falling in the respective
regions.
Recommended Use
We recommend you review the standard curve fit optimizer report after
running an optimization, and review the Results and Comments material
outlined below. These are designed to help you review your data quickly for
issues that may concern you. We also recommend that you pay particular
attention to those analytes with comments, or those that return a Check Data
state.
BioPlex_6.book Page 201 Friday, September 23, 2011 11:55 AM