Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 238)

Bio-Plex Manager Software 6.1 User Guide | Appendix
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Ratio (Simple) and the Grouping Function
If you are not using the grouping function present in the plate formatting
section of the software, this does not apply to you.
When using this function, remember that the Ratio value presented is only
relevant for making comparisons within a group. Outside the group, these
numbers are arbitrary and meaningless (unless the reference sample is
another instance of the exact same biological sample in every group).
Since data outside of groups cannot be compared, the grouping function in
the plate formatting section of the software defeats any inter- and many intra-
experiment normalization. Relative differences among samples within the
same group are still valid.
If you are normalizing your data, ratio calculation is a simple ratio of the
calculated value of a reference sample of the group, compared to the
calculated value of each member of that group. Data can be presented as
member over reference, or as reference over member.
Ratio =
If you are not normalizing your data, the ratio is calculated using FI values.
Ratio =
BioPlex_6.book Page 226 Friday, September 23, 2011 11:55 AM