Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 106)

Bio-Plex Manager Software 6.1 User Guide | Preparing Protocols
94
L OGISTIC W EIGHTING
If you select a logistic curve fitting method (Logistic-5PL or Logistic-4PL), the
software automatically weights the points in the curve. Click the Logistic
Weighting button to access the weighting algorithm controls.
Figure 86. Logistic Weighting dialog
There are eight models for calculating the variance in the logistic weighting
algorithm:
• Linear Variance
• Logarithmic Variance
• Exponential Variance
• Power Law Variance (default)
• Linear CV
• Logarithmic CV
• Exponential CV
• Power Law CV
C OEFFICIENT A
Weighting coefficient a will be calculated automatically using the following
equation:
a = .002 * pow ((m_YMax + m_YMin) / 2 + blank,.2)
Alternatively, you can enter your own value by deselecting the Auto calculate
coefficient a checkbox and entering a value in the Coefficient a field.
C OEFFICIENT B
Weighting coefficient b has a default value of 1.8. You can enter a different
value in the Coefficient b field.
BioPlex_6.book Page 94 Friday, September 23, 2011 11:55 AM