Assigning a control sample – Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
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Figure 192. Selecting Multiple Internal Controls
Double-click an analyte, or select and click Add. The housekeeping genes
populate the Selected field of the dialog box. Bio-Plex Manager™ allows you
to add up to 10 housekeeping genes.
NOTE: The normalized value for each sample is calculated by dividing the
mean fluorescence intensity (MFI) of the analyte of interest by the MFI of the
analyte used for normalization. When multiple analytes are used for
normalization, the geometric mean of the normalization analytes' MFI is used.
Assigning a Control Sample
The Control Sample field in this dialog allows you to express all gene sample
measured values relative to an assigned control. The control sample has a
value of 1 and other samples are expressed as ratios (for example, 5.57-fold
higher mRNA compared to the control, or 2-fold higher protein level compared
to the control).
Brackets around analytes in any list indicate they are used as internal controls.
To assign a control sample, click the Single button in the Control Sample field.
Click None to disable your choice.
BioPlex_6.book Page 209 Friday, September 23, 2011 11:55 AM