9 analyzing the results, Results files, Chapter 9. analyzing the results – Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual

Page 155: Analyzing the results

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Bio-Plex Manager Software 6.1 User Guide

143

9

Analyzing the Results

Results Files

After a reading is complete, a Results file generates and opens automatically.
The Results file contains the data from a reading, the Protocol parameters
used to collect that data, and analysis tools for interpreting the data. It
includes all the information necessary to analyze raw data, including a
description of the assays, well types, concentrations, dilutions, and the
regression method used for calculating the concentrations of unknowns from
standards. It also includes information about the instrument and the run
conditions at each well at the time of data acquisition.

In the Results file, you can change many of the parameters copied from the
original Protocol file—including the plate format, analyte selection, standards
info, etc.—and recalculate the results. However, you can change only the
parameters of wells that have already been read, and you can calculate only
results based on the analytes that were present in the sample.

NOTE: If you selected Auto Save After Run in the Protocol window, the
Results file is automatically saved after a reading. Otherwise, you must save
your Results file as a separate step.

To generate a Results file from an incomplete reading or a previous reading,
open the Protocol file that contains the raw data for that reading and select

New Results from Protocol

from the Run Protocol toolbar or the File

menu.

BioPlex_6.book Page 143 Friday, September 23, 2011 11:55 AM

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