Bead map – Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
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Histogram and Bead Map
115
Click the Maximize button
to maximize the histogram portion of the
window within the larger Protocol window. Click the Restore button
to
return the histogram to its default state.
Figure 103. Histogram maximized in the Protocol window
Bead Map
The bead color map is a density dot plot of the events in a reading for a
selected well. Different colored dots in the map represent different numbers of
events at those data points. The number of available regions in the bead map
(25 or 100) is listed above the map, and is based on the selection in the
Advanced Settings dialog (see page 104).
In the default bead map, the x-axis is the Classification 1 channel and the y-
axis is the Classification 2 channel. These channels measure the embedded
fluorochromes in each bead, which are used to identify the bead set and
corresponding analyte. The resulting data point clusters in the map represent
the different bead sets in the assay and their associated analytes.
NOTE: Each bead set generates a cluster of data points, rather than a single
point, because of minute variations in fluorochrome levels and intensity
among the beads in a set.
BioPlex_6.book Page 115 Friday, September 23, 2011 11:55 AM