Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 225)

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The following table is based on the selections made in Normalization Options.
It also describes how the Norm (Ratio) is calculated:
NOTES:
• Replicates are done at the plate level. See the section on plates to
define replicates
• When using multiple replicates, the Norm (Ratio) for each replicate
is calculated as described in Case A, B, C, D, and E. For each
sample type, the replicates' average is the Norm (Ratio)
• For information about the formulas Bio-Plex Manager uses to
calculate normalization values, see Normalization Formulas on
page 223
Case
Internal
Controls Control
Sample
How Norm (Ratio) is Calculated
A Single None Ratio of (FI-Bkgd) for the analyte and the
housekeeping gene:
Norm (Ratio) = (FI-Bkgd)/(FIhk-Bkgdhk)
B Multiple None Geometric mean of the normalized (FI - Bkgd)
ratios for each housekeeping gene
C Single Single Normalized (FI - Bkgd) ratio/(FI - Bkgd) control
ratio for the control (as defined in Case A):
Norm (Ratio) = Norm (Ratio) single gene/Norm
(Ratio) control
D Multiple Single (Ratio) multiple internal control divided by the
Norm (Ratio) multiple control (as defined in
Case B) for the control:
Norm (Ratio) = Norm (Ratio) multiple internal/
Norm (Ratio) multiple…
E None Single Ratio of (FI-Bkgd) for the analyte and the
control:
Norm (Ratio) = (FI-Bkgd)/(FI control-
Bkgdcontrol)
BioPlex_6.book Page 213 Friday, September 23, 2011 11:55 AM