Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 212)

Bio-Plex Manager Software 6.1 User Guide | Background
200
Refer to Determining Outliers on page 201 for more information and
instructions.
Background
Standard Curve Recovery
Bio-Plex Manager calculates an observed concentration for each standard
point from the standard curve. This is divided by the expected concentration
(entered by the user in the Enter Standards Info window) and multiplied by 100
to give a recovery percentage. These calculations are automatically performed
by the software. The reported recovery of the standard curve points are in the
Obs/Exp * 100 column pictured below.
Figure 185. Obs/Exp * 100 column reports Standard Curve Recovery
The recovery percentages of the standard curve points indicate the overall
accuracy of the assay. The user can specify more or less stringent ranges for
individual assays.
Use the Acceptable Recovery Range drop-down menu in the Enter Standards
Info window to select an acceptable recovery range. To set different recovery
ranges for different analytes, uncheck the box next to Same recovery range
for all analytes. For more information on this feature, see Recovery
Percentage Range on page 95.
BioPlex_6.book Page 200 Friday, September 23, 2011 11:55 AM