Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 243)

Microsphere Handling
231
Microsphere Agitation During Assay
Agitation of Bio-Plex assays with a plate shaker during incubation steps
prevents microspheres from settling during extended incubation periods. The
benefit of agitation may not be discernible for some assay conditions and
should be evaluated according to the requirements of a given application. It is
important to resuspend the beads in an assay for 30 seconds before
performing a reading.
Microsphere Stability and Storage
xMAP microspheres are light sensitive and should be protected from light
during all stages of storage and usage. In addition, freezing conditions and
organic solvents should be avoided.
BioPlex_6.book Page 231 Friday, September 23, 2011 11:55 AM