Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 72)

Bio-Plex Manager Software 6.1 User Guide | Preparing Protocols
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NOTE: During a reading, the array reader always detects all the analytes in the
sample, including any you have not selected. However, analytes that were
detected but not selected will, by default, not appear in the final reports and
tables. After a reading, you can go back and correct your selections in the
Protocol window and/or the Results file, and the detected analytes will appear
in the tables.
Click the Select Analytes button to view the analyte selection window.
Figure 41. Select Analytes window
Bio-Plex Manager groups analytes by panels. Preconfigured panels of
analytes are built into the software; these panels correspond to off-the-shelf
Bio-Plex assays, and include human, mouse, and rat cytokines and
phosphoproteins, plus the newer human angiogenesis, diabetes, isotyping
and acute phase assays. You can add to these existing panels of analytes, or
create new panels that contain only the analytes you use in your experiments.
Bio-Plex cytokine, phosphoprotein, and total target assays are available as
custom-mixed x-Plex™ assays. For repeat sample testing, these multiplex
assays are offered in one or ten 96-well plate formats. Select from the current
list of available assays, and Bio-Rad will mix and quality-test the coupled
beads and detection antibodies to order. You can obtain product information
and a catalog number for your custom-designed x-Plex assay by going to
www.bio-rad.com/bio-plex/x-plex .
First select the panel of analytes that you want reported, then select the
specific analytes.
S ELECTING A NALYTES
Go to the Panel pulldown menu and select from the list of preconfigured
panels. The analytes in the selected panel display in the Available list.
BioPlex_6.book Page 60 Friday, September 23, 2011 11:55 AM