Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 107)

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R ECOVERY P ERCENTAGE R ANGE
Because the standard curve (page 168) is critical for calculating the
concentrations of your unknowns, Bio-Plex Manager includes a mechanism
for assessing the fit of the curve to the standards. This is the recovery
percentage. See Standard Curve Optimizer on page 199 for how Bio-Plex
Manager incorporates Recovery Range data automatically.
For each analyte standard, an observed concentration is back-calculated from
the standard curve and the fluorescence intensity. This is divided by the
expected concentration as entered in the Enter Standards Info window and
multiplied by 100 to give the recovery percentage:
Recovery percentage = Observed conc/Expected conc * 100
Recovery percentages are also used with controls (page 96) to determine the
overall accuracy of an assay.
In the Enter Standards Info window, use the Acceptable Recovery Percentage
Range pulldown menu to select an acceptable recovery range.
Figure 87. Selecting the recovery percentage range
For example, if you select a range of 70 to 130%, the observed concentration
should be within 70 to 130% of the expected concentration.
The actual recovery rate for each standard and control is shown in the Obs/
Exp * 100 column of the Report table (see page 160). Unknown
concentrations that fall in regions of the curve where standards fall outside the
selected range will be reported as OOR< or OOR> in the Conc in Range
column of the Report table. (See Report Table Error Codes on page 234.)
BioPlex_6.book Page 95 Friday, September 23, 2011 11:55 AM