Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 172)

Bio-Plex Manager Software 6.1 User Guide | Analyzing the Results
160
Obs/Exp * 100 Column
Because the standard curve (see page 168) is critical for calculating the
concentrations of unknowns, the Obs/Exp * 100 column in the Report Table
allows you to assess how well the curve fits the standards. This column is also
used with controls (page 96) to determine the overall accuracy of an assay.
The (Obs/Exp) * 100 column shows the recovery rate for the observed vs.
expected concentration of each standard. This number should fall within the
recovery percentage range selected in the Enter Standards Info window. For
example, if you selected a recovery range within 80 to 120%, the observed
(back-calculated) concentration of a standard should be within 80 to 120% of
the expected concentration. Values outside this range are flagged in the Conc
in Range column.
Click Report Table Options and select the (Obs/Exp) * 100 checkbox to
display this column.
Concentration in Range Column
The concentration in range column (Conc in Range) provides a method for
determining which unknown concentrations are reliable based on the recovery
range of the standards (see the Obs/Exp * 100 section above).
This column shows only observed concentration values that fall within the
range of valid standards; values that do not fall in the range are displayed as
OOR< or OOR>. (The concentration in range values of the back-calculated
standards are also shown, for comparison purposes.)
Figure 142. Concentration in Range column
Back-calculated standard
concentrations within a specified
recovery percentage range of
80–120%.
Concentrations of unknowns
within range
Concentrations of unknowns
outside range
BioPlex_6.book Page 160 Friday, September 23, 2011 11:55 AM