Normalization definitions, Calculations based upon your settings – Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
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Bio-Plex Manager Software 6.1 User Guide | Data Normalization
212
Normalization Definitions
The following columns and their associated calculations are provided when
performing data normalization. Here are definitions for these columns:
•
Norm Ratio—normalized ratio. See Normalization Formulas on
page 223, for details about the formulas used for this calculation
•
Norm Ratio Std Dev—standard deviation of the Norm Ratio values
for all the wells within a sample's replicate group
•
Norm Ratio Std Err—the normalized standard deviation, Norm
Ratio Std Dev, divided by the square root of the number of replicate
wells within the sample's replicate group
•
Norm Ratio %CV—the normalized standard deviation, Norm Ratio
Std Dev, divided by the normalized ratio of the sample's replicate
group
•
Norm FI—normalized fluorescent intensity. See Normalization
Formulas on page 223, for details about the formulas used for this
calculation
•
Norm FI Std Dev—standard deviation of the Norm FI values for all
the wells within a sample's replicate group
•
Norm FI Std Err—the normalized standard deviation, Norm FI Std
Dev, divided by the square root of the number of replicate wells
within the sample's replicate group
•
Norm FI %CV—the normalized standard deviation, Norm FI Std
Dev, divided by the normalized FI of the sample's replicate group
Calculations Based Upon Your Settings
Norm (Ratio)—Normalized (FI—Bkgd) ratio.
BioPlex_6.book Page 212 Friday, September 23, 2011 11:55 AM