Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 51)

Remove Air Bubbles
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Remove Air Bubbles
Microscopic air bubbles in the cuvette may cause a sudden shift in the bead
regions during an assay reading. If the array reader detects such a shift, the
reading stops and you are prompted to perform an alcohol wash to force air
bubbles out of the system.
Click the Remove Bubbles button on the main toolbar or select the
command from the Instrument menu. A dialog box guides you through the
steps for preparing the MCV Plate IV for the procedure.
Figure 23. Steps for removing air bubbles from the fluidics system
Add 70% isopropanol and distilled water to the appropriate reservoirs, insert
the plate in the microplate platform, and click OK. This procedure performs a
series of fluidics operations and takes several minutes. After initial system
pressurization (5 to 20 seconds), the time remaining in the operation displays
in the Bio-Plex Manager status bar.
BioPlex_6.book Page 39 Friday, September 23, 2011 11:55 AM