Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 248)

Bio-Plex Manager Software 6.1 User Guide | Glossary
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Concentration in range A measure of unknown, standard, and control concentrations that are
reliable, based on the recovery range of valid standards. Values outside
the range are flagged as >OOR or <OOR.
Conjugated
microsphere A microsphere set that has been chemically modified so that its surfaces
are covered with molecules that bind with the target analytes in an assay
system.
Cytokine An immunological protein that can signal a response in the immune
system.
Detection antibody An antibody used in capture sandwich immunoassays that binds a
captured analyte to a fluorochrome, allowing for quantitation of the
protein. Detection antibodies may be biotinylated, which allows for
addition of a streptavidin-bound fluorochrome, or the detection antibody
may be directly labeled with a fluorochrome.
Doublet Two microspheres that have associated. The resulting signal of the
associated microspheres is twice that of a single microsphere (singlet).
Doublet discriminator A channel that measures the amount of light scatter from particles that
flow past the red laser. Light scatter is directly proportional to particle
size, and the channel is designed to identify particles that are smaller or
larger than a single microsphere, including microspheres that are
clumped or aggregated.
Dynamic range The calculated number of decades covered by the reporter channel
scale of the array reader. Range is calculated using the Reporter
Validation Kit.
Emission spectrum The wavelength range of light emitted by an excited fluorochrome when
its electrons fall from a higher to a lower energy state. Expressed in
nanometers (nm).
Ex/Em A common way of reporting a molecule's excitation and emission
wavelengths (for example, Ex/Em = 488/520 nm).
Excitation spectrum The wavelength range that excites a molecule's electrons to a higher
energy state. Expressed in nanometers (nm).
External standard A standard that has been imported from a different file.
Fit probability A statistical measure of goodness of fit that incorporates the residual
SSE (sum of the square of the error). A fit probability of 1 indicates a
perfect fit, while a fit probability of 0 indicates no fit.
Fluidics validation The process of verifying the well-to-well carryover of beads in the array
reader.
Fluorescence The emission of light that occurs when the electrons of a fluorochrome
drop to a lower energy state.
Fluorochrome A fluorescent molecule.
Gate An electronic method of discriminating or eliminating aggregated
microspheres in the analysis of an assay. A gate separates singlet beads
from aggregated beads.
Term Definition
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