Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 132)

Bio-Plex Manager Software 6.1 User Guide | Running Protocols
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Bead Statistics
To display statistical data for the individual beads as well as the entire well,
click the Show/Hide Bead Statistics Columns button on the table
toolbar. The number of beads collected for each analyte displays in
parentheses after the analyte fluorescence intensity.
Figure 108. Analyte fluorescent intensity and bead count
With Show/Hide Bead Statistics Columns selected, the following columns are
also displayed:
• Total—total bead count in the well
• Region—number of beads that fell within defined regions
• Gate—number of beads that passed the DD gate (see page 107)
• % Agg Beads—percentage of aggregated beads in the well
calculated as (100 – [Gate/Total*100])
Figure 109. Well data
NOTE: The number in the Region column is flagged by an asterisk (*) if that
well did not acquire the number of beads per region specified for the reading.
Sampling Error Codes
During a reading, the flow of beads, bead count, bead regions, and platform
temperature are continuously monitored. The Sampling Errors column reports
errors in any of these areas for each analyte. These errors are reported in the
table even if you chose not to pause the run due to an error condition (see
page 105).
The sampling error codes in the column are listed in the following table.
Error Code Indication
1 Low bead number detected in the well
2 Aggregated beads detected in the well
BioPlex_6.book Page 120 Friday, September 23, 2011 11:55 AM