Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 46)

Bio-Plex Manager Software 6.1 User Guide | Controlling the System
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Performing the Calibration
Click the Eject/Retract Plate button to eject the microplate platform plate
carrier. Place the MCV Plate IV in the carrier with the arrow facing toward the
platform, and then retract the plate. Click OK to start the calibration process.
As calibration proceeds, the status bar at the bottom of the Bio-Plex Manager
window monitors the progress of calibration. The number of beads per
second should be 100 or higher. Fewer beads per second may indicate a
problem with the fluidics system.
Figure 20. Status bar during calibration
Because calibration beads are highly concentrated, calibration is followed by
three wash cycles using distilled water.
An alert box notifies you whether calibration has succeeded or failed. You also
receive an alert which informs you how many days have elapsed since you
last ran a validation procedure.
If the calibration process failed, check the MCV Plate IV to verify that the
correct beads were added to the wells. Then perform a fluidics wash and
repeat the above steps. For more information, consult the Troubleshooting
chapter.
Logging the Calibration
The Calibration Log provides a list of past calibration dates and times, results,
instrument settings, and other data obtained by Bio-Plex Manager. These data
are stored in a log file called bioplexdata.mdb. By default, the file is saved in
the main Bio-Plex Manager application folder on your computer.
NOTE: This database is not compatible with versions of Bio-Plex Manager earlier
than 4.0. If you have an earlier version of Bio-Plex Manager, installing version 4.0
or later copies data from your existing database (bioplex.mdb) into the new
database. A copy of the old database remains in the application folder.
Number of beads per second should be 100 or higher
BioPlex_6.book Page 34 Friday, September 23, 2011 11:55 AM