Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
Page 128
Bio-Plex Manager Software 6.1 User Guide | Running Protocols
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The white areas on the map indicate the expected regions of the selected
analytes. If you move your cursor over these areas, the name of the analyte
appears in a pop up window. The data point clusters should fall within these
regions.
Figure 104. Bead map, with pop-up window indicating the analyte region
under the cursor
If one or more clusters do not fall within the white areas, there may be a
problem with the beads such as photobleaching, or the analyte selections in
the Protocol may be incorrect. If all the data clusters have shifted out of the
white regions, there may be microbubbles in the cuvette. See the
Troubleshooting chapter on page 215 for troubleshooting suggestions.
In this way, the map can be used to confirm that the bead sets are correctly
selected, measured, and identified.
C
HANGING
THE
X
-
AND
Y
-
AXES
OF
THE
M
AP
You can change the channel types of the axes. Right-click the bead map and
select one channel from the x-axis submenu, and the other channel from the
y-axis submenu. The available channels are:
•
Doublet Discriminator —This channel measures light scatter from
the beads as they pass through the red laser. Light scatter
correlates to particle size
•
Reporter 1 —This channel measures the reporter molecules bound
to the analytes on each bead
•
Classification 1—This channel measures the first classification dye
embedded in each bead
•
Classification 2—This channel measures the second classification
dye embedded in each bead
BioPlex_6.book Page 116 Friday, September 23, 2011 11:55 AM