Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 252)

Bio-Plex Manager Software 6.1 User Guide | Glossary
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Slope Defined as the rise over the run when plotting a series of points to form a
line. Using the Reporter Validation Kit, the slope of the response is
related to the dynamic range of the array reader and yields information
about the response of the photomultiplier tube (PMT).
Spectral address The unique fluorescent emission spectra of a microsphere.
Standard deviation The spread in individual data points (for example, in a replicate group) to
reflect the uncertainty of a single measurement.
Standard error The standard deviation of a set of replicates divided by the square root
of the number of replicates.
Unconjugated
microspheres A microsphere set that has been chemically modified so that its surfaces
are covered with some functional group, but that has not been
chemically prepared for use as a component in an assay system. These
base microspheres do not carry biomolecular reactant.
Uniformity The reproducibility of a signal over a series of replicate measurements.
Upper Limit of
Quantitation For any given assay, this is the highest value Bio-Plex Manager will
report in the Concentration in the Range column of the data table.
Values above this range are considered less trustworthy based on either
the poor recovery values of standards above this range or saturation of
the assay. For more details, review the definition of Concentration in
Range.
Validation A formal process for documenting that an instrument is fit for its
intended use and is kept in an appropriate state of maintenance and
calibration.
Validation kit A set of reagents and procedures designed to verify the performance of
the array reader apart from an assay. Bio-Plex Validation Kit 4.0
assesses the alignment of the optics assembly, measures the
performance of the reporter channel, evaluates the fluidics system, and
verifies the classify efficiency of the array reader.
Variance The mean square deviation of a variable around the average value.
Term Definition
BioPlex_6.book Page 240 Friday, September 23, 2011 11:55 AM