Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 161)

Results Files
149
Changing the Doublet Discriminator Gate
Range
You can change the doublet discriminator (DD) gate range (see page 107) in
the Results file and recalculate the data based on the new range. The original
DD gate range set in the Protocol file and used during the reading is preserved
and can be displayed in the Raw Data table.
To reset the DD gate range, go to the Edit menu, open the Reanalyze Results
submenu, and select Change DD Gates. The Change DD Gates dialog opens,
displaying the histogram and bead map with controls for changing the DD
gate range. (See page 111 for a complete description of other histogram and
bead map controls.)
Figure 132. Change DD Gates dialog.
In the Change DD Gates dialog, first select the well you want to change or
select the Same DD Gates for All Formatted Wells checkbox. Also, you can
select a specific analyte, all gated analytes, or all analytes from the pulldown
menu above the histogram. Then either drag the red dashed lines in the
histogram display to adjust the DD gates, or enter new values in the DD Low
and/or DD High fields. Click the Update Gates button.
When you have made your changes, click OK. The data in the Raw Data table
(see next section) recalculate based on the new settings.
BioPlex_6.book Page 149 Friday, September 23, 2011 11:55 AM