Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 80)

Bio-Plex Manager Software 6.1 User Guide | Preparing Protocols
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Step 3. Format Plate
After you have selected analytes, you are ready to specify the format of your
96-well microtiter plate using the plate template in the Protocol window. The
formatting in the plate template tells the array reader which wells to read, and
tells Bio-Plex Manager how to analyze the different sample types in each well.
NOTE: Only formatted wells will be read by the array reader. Be careful to
format all the wells that you want to read before running a protocol.
Click the Format Plate button in the Protocol window to display the plate
template and the formatting controls.
Figure 53. The plate template and formatting toolbar (Plate Formatting view)
The Format Plate window has two views: the Plate Formatting view and the
Plate Groupings view. You can toggle between the views using the tabs above
the plate diagram. As you toggle, the plate template remains the same, but the
commands toggle between formatting controls and grouping controls.
• Plate formatting tools are used to define the types of wells in a
plate (sample, control, standard, blank, etc.)
• Plate groupings tools are used to organize the well types into
groups, with one member of each group defined as the Reference
(or Primary) member, as in a Western blot kinase assay. The ratio of
each member's fluorescent intensity to the fluorescent intensity of
the Reference can then be calculated
NOTE: Plate formatting is required to perform a reading, because the array
reader will read only formatted wells, whereas plate groupings are optional
and can be defined later.
BioPlex_6.book Page 68 Friday, September 23, 2011 11:55 AM