Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 87)

Protocol Window
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Plate Groupings
After you have formatted the wells (see previous section), you can organize
them into groups and identify one member of each group as the Reference, or
Primary member. You can then calculate the ratio of each member's
fluorescence intensity to the fluorescence intensity of its Reference. This type
of analysis is similar to that performed in Western blotting.
Click the Plate Groupings tab to display the plate grouping tools.
Figure 64. Plate Groupings view
The buttons on the toolbar are used to group the wells on the microtiter plate
diagram and identify the Reference well(s). These formatting commands are
also located on the Format Options menu.
Figure 65. Tools in the Plate Groupings view
First, select the ratio you want to calculate for each group — Reference/
Member or Member/Reference — using the Ratio field pulldown list.
BioPlex_6.book Page 75 Friday, September 23, 2011 11:55 AM