Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 230)

Bio-Plex Manager Software 6.1 User Guide | Troubleshooting
218
The calibration was
unsuccessful. Please
repeat calibration. If
calibration fails a
second time, follow
these steps.
Or
The calibration was
unsuccessful. Bio-Plex
Manager has detected
a problem with low
bead number. Please
repeat calibration.
Calibration procedure failed.
Or
Calibration procedure failed due
to low bead number.
1. Make sure CAL1 beads and CAL2
beads are placed in the appropriate
wells (CAL1 in red well and CAL2 in
green well).
2. Make sure the calibration target
values match the target values printed
on the CAL1 and CAL2 bottles.
3. Replace or clean the needle.
4. Adjust needle height.
5. Run Unclog until it passes.
(If it doesn't pass by the 5th attempt,
call Technical Support.)
6. Be sure to calibrate with freshly
vortexed beads and a clean MCV IV
plate.
7. Repeat calibration and record bead
flow (optimum flow should be > 200
beads/second)
If problem persists, record any
additional error messages (such as
temperature problem, pressure
fluctuation, warm-up cancellation)
before calling tech support.
Optics Validation
Procedure shows
value(s) outside of
acceptable range(s). Problem with optical component
of array reader. Repeat validation procedure. If values
are still out of range, contact Technical
Support.
Fluidics Validation
Procedure shows
value(s) outside of
acceptable range(s). Problem with fluidics component
of array reader. Repeat validation procedure. If values
are still out of range, contact Technical
Support.
Reporter Validation
Procedure shows
value(s) outside of
acceptable range(s). Problem with optical component
of array reader. Repeat validation procedure. If values
are still out of range, contact Technical
Support.
Classify Validation
Procedure shows
value(s) outside of
acceptable range(s). Problem with calibration or
optical component of array
reader. Repeat validation procedure. If values
are still out of range, contact Technical
Support.
Message/Problem Cause Solution
BioPlex_6.book Page 218 Friday, September 23, 2011 11:55 AM