Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 135)

Rerun/Recovery Mode
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Rerun/Recovery Mode
After a reading has stopped—because of an error or user intervention, or
because the reading is complete—the Rerun/Recovery Mode checkbox in the
Run Protocol window will be selected. This allows you to rerun some or all of
the samples.
With Rerun/Recovery Mode selected, checkboxes appear next to each well
number in the Run Protocol table. Select the checkboxes next to the wells you
want to read again.
Figure 112. Selecting wells to rerun
NOTE: To rerun wells in a filter plate, you must first vacuum the wells using the
vacuum filter apparatus. The beads remain on top of each filter plug after
vacuuming. Resuspend the beads with 125 μl of resuspension buffer in each
well. See the Bio-Plex Cytokine Assay Manual for detailed instructions.
Each well may be read twice without adversely affecting the results.
Because there are fewer beads in a well that has already been run once, be
sure to select 100 beads per region or fewer in the Run Protocol settings (see
page 101). Also, click the Advanced Settings button and deselect the Low
bead number error condition checkbox (page 104). Otherwise, a low bead
error may stop the reading.
Click Start to begin the reading.
BioPlex_6.book Page 123 Friday, September 23, 2011 11:55 AM