Bio-Rad Bio-Plex Software® Upgrades and Conversions - Manual (Page 249)

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Group A group of wells defined on a plate. You can calculate the ratio of the
fluorescent intensities of the group's member wells to the fluorescent
intensity of a Reference well in the group.
Immunofluorescence A technique that uses a covalently linked fluorochrome-antibody
complex to detect or quantify a particular antigen.
Instrument threshold The instrument threshold is a measure of system noise, and is the
reporter channel signal from a blank bead that contains no reporter dye.
Kinase An enzyme that functions to phosphorylate specific proteins. Kinases are
responsible for the activation of a variety of proteins through the process
of phosphorylation.
Laser A highly purified source of light used to excite fluorochrome electrons.
An acronym for Light Amplification by Stimulated Emission of Radiation.
Linearity A measurement of the coefficient of determination, or R 2 value, of a set
of standard beads. An R
2
value of 0.995 or greater is an acceptable
linearity value using the Bio-Plex Validation Kit.
Logistic regression A regression model for binary (dichotomous) outcomes. The data are
assumed to follow binomial distributions with probabilities that depend
on the independent variables.
Lower limits of
Quantitation For any given assay, this is the lowest value Bio-Plex Manager will report
in the Concentration in Range column of the data table. Values below
this range are considered less trustworthy based on either the poor
recovery values of standards below this range or background of the
assay. For more details, review the definition of Concentration in Range.
Median fluorescent
intensity A relative measurement of fluorescent intensity based on the median, a
robust statistical measure.
Microparticle A solid substance with a diameter in the micrometer range. Often used
as a synonym for a microsphere.
Microsphere set A set of multianalyte microspheres containing a unique mixture of two
distinct fluorochromes to distinguish them from other multianalyte
microspheres.
Microspheres Latex spheres with a diameter in the micrometer range. Also called
beads.
Multianalyte or multiplex An analysis of several assays or tests performed simultaneously in the
same reaction container.
Operational qualification The process of determining that an instrument is fit for its intended use.
The Bio-Plex Validation Kit is a tool for operational qualification.
Optics validation The process for verifying the alignment of the optics assembly of the
array reader.
Outlier A well or replicate group of wells whose measured value is outside the
normal range. You can exclude outliers from statistical calculations.
Panel A group of related bead sets. For example, the Human Cytokine panel
includes bead sets for Human IL-2, Human IL-4, Human IFN gamma,
and other human cytokines. You can select multiple bead sets from the
same panel or sets from different panels in the same multiplex assay.
Term Definition
BioPlex_6.book Page 237 Friday, September 23, 2011 11:55 AM