Components – Bio-Rad Bio-Plex Software® Upgrades and Conversions User Manual
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Bio-Plex Manager Software 6.1 User Guide | Components
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The system also includes validation and calibration reagents, a selection of
cytokine and phosphoprotein assays, sample preparation reagents, and a
computer running Bio-Plex Manager
™
6.1 software. New angiogenesis, acute
phase, diabetes, cancer and isotyping assays have been introduced with this
version of the software.
Check
www.bio-rad.com/bio-plex/x-plex
for newly-available assays and
reagents.
The assays contain sets of microscopic color-coded beads, each of which is
conjugated with a different reactant.
Reactants can include:
•
DNA
•
Enzyme substrates
•
Receptors
•
Antigens
•
Antibodies
These can be used to create, for example, a capture sandwich immunoassay.
To perform a multiplex reading, samples are mixed with conjugated
microsphere and reactant mixtures; next, fluorescent reporter molecules are
added. The assays are loaded into the wells of a 96-well microtiter plate, and
the plate is inserted into the microplate platform. The platform and array
reader are controlled by a computer running Bio-Plex Manager software.
The detection system in the array reader uses two lasers to analyze the
microspheres in a flow stream. The first laser identifies each microsphere and
its associated analyte based on the fluorescent signature of the microsphere,
and the second measures the amount of analyte, using the reporter molecules
attached to the analytes. When the reading is complete, Bio-Plex Manager
displays the raw data and generates detailed summary reports.
Components
A certified Bio-Rad service engineer will install the complete Bio-Plex
suspension array system at your site, including the array reader, microplate
platform, and computer. The system setup procedure is described in
“Bio-Plex 200 System Hardware Instruction Manual”, available for download
from the Bio-Rad website.
The Bio-Plex suspension array system includes the following components:
•
Array reader
•
Microplate platform
•
Bio-Plex MCV Plate IV (required for Bio-Plex Manager 5.0 and later)
BioPlex_6.book Page 2 Friday, September 23, 2011 11:55 AM